bio rad chemidoctm imaging system (Bio-Rad)
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bio rad chemidoctm imaging system
Bio Rad Chemidoctm Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+chemidoctm+mp+imaging+system/ChemiDoc+MP+Imaging+System/pm42105149-71-7-7
Average 99 stars, based on 22840 article reviews
Bio Rad Chemidoctm Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+chemidoctm+mp+imaging+system/ChemiDoc+MP+Imaging+System/pm42105149-71-7-7
Average 99 stars, based on 22840 article reviews
bio rad chemidoctm imaging system - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: RIPK1-driven calcium overload and lysosomal-mitochondrial dysfunction induce testicular necroptosis following DEHP exposure. Article Snippet: Following a 10% nonfat milk blocking step, the membranes were incubated with primary antibodies against phosphorylated RIPK1 (p-RIPK1) (Cell Signaling Technology, 53286, 1:1000, USA), phosphorylated MLKL (p-MLKL) (Affinity, AF7420, 1:1000, China), phosphorylated RIPK3 (p-RIPK3) (affinity, AF7443, 1:1000, China), RIPK1 (Proteintech, 17519–1-AP, 1:750, China), RIPK3 (Proteintech, 17563–1-AP, 1:750, China), MLKL (Affinity, DF7412, 1:1000, China), cathepsin B (CTSB) (Cell Signaling Technology, 31718, 1:1000, USA), calpain 1 (Proteintech, 10538–1-AP, 1:750, China), TNF-α (Proteintech, 17590–1-AP, 1:750, China),TNFR1 (Proteintech, 21574–1- AP, 1:750, China),Cytochrome c (Cyt c) (Proteintech, 10993–1-AP, 1:2000, China),zonula occludens-1 (ZO-1) (Proteintech, 21773–1-AP, 1:5000, China), connexin-43 (Cx-43) (Proteintech, 26980–1-AP, 1:1000, China), PGAM5 (Affinity, DF13355, 1:1000, China), β-actin (Aroteintech, 66009–1-Ig, 1:5000, China), COX IV (Wanleibio, WL02203, 1:1000, China) at 4°C overnight (Table S2). .. The membranes were incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies at room temperature for 2 h. The bound antibody was detected by an ECL kit and Article Title: Phage display-mediated immuno-PCR to detect low-abundance secreted proteins in Drosophila . Article Snippet: To detect the VHH05 or 127D01 epitopes, NbVHH05- human IgG or Nb127D01- human IgG (1:100, S2 cell culture media) and Goat anti- Human IgG- HRP (1:5,000, Thermo Fisher Scientific, A18829) were used. .. The incubated antibodies were washed with TBST for 20 min, and signals were detected with enhanced chemiluminescence (Thermo Fisher Scientific, 34095) and Article Title: Circadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult Mice Article Snippet: To quantify DNA loading, the same samples were loaded to the other half of the membranes and probed with anti-ssDNA antibody (MAB3034, Millipore Sigma, Burlington, MA, USA) with 1/15,000 dilution in 0.2% Tween-20 (PBS-T). .. After being incubated overnight at 4 ◦C, these were washed with PBS-T, and incubated in secondary antibody, Rabbit-anti-mouse IgG (cat. No. ab46540, Abcam, Cambridge, UK), at room temperature for 1 h. After washing, membranes were developed https://doi.org/10.3390/ijms27083408 using the Bio-Rad Western ECL kit and imaged on a Imaging:Article Title: RIPK1-driven calcium overload and lysosomal-mitochondrial dysfunction induce testicular necroptosis following DEHP exposure. Article Snippet: Following a 10% nonfat milk blocking step, the membranes were incubated with primary antibodies against phosphorylated RIPK1 (p-RIPK1) (Cell Signaling Technology, 53286, 1:1000, USA), phosphorylated MLKL (p-MLKL) (Affinity, AF7420, 1:1000, China), phosphorylated RIPK3 (p-RIPK3) (affinity, AF7443, 1:1000, China), RIPK1 (Proteintech, 17519–1-AP, 1:750, China), RIPK3 (Proteintech, 17563–1-AP, 1:750, China), MLKL (Affinity, DF7412, 1:1000, China), cathepsin B (CTSB) (Cell Signaling Technology, 31718, 1:1000, USA), calpain 1 (Proteintech, 10538–1-AP, 1:750, China), TNF-α (Proteintech, 17590–1-AP, 1:750, China),TNFR1 (Proteintech, 21574–1- AP, 1:750, China),Cytochrome c (Cyt c) (Proteintech, 10993–1-AP, 1:2000, China),zonula occludens-1 (ZO-1) (Proteintech, 21773–1-AP, 1:5000, China), connexin-43 (Cx-43) (Proteintech, 26980–1-AP, 1:1000, China), PGAM5 (Affinity, DF13355, 1:1000, China), β-actin (Aroteintech, 66009–1-Ig, 1:5000, China), COX IV (Wanleibio, WL02203, 1:1000, China) at 4°C overnight (Table S2). .. The membranes were incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies at room temperature for 2 h. The bound antibody was detected by an ECL kit and Article Title: Photobiomodulation at 830 nm with 5 J/cm 2 does not promote PI3K/AKT/mTOR signalling pathway activation in hyperglycemic wounded cells Article Snippet: .. The bands presence were scanned using a Article Title: Phage display-mediated immuno-PCR to detect low-abundance secreted proteins in Drosophila . Article Snippet: To detect the VHH05 or 127D01 epitopes, NbVHH05- human IgG or Nb127D01- human IgG (1:100, S2 cell culture media) and Goat anti- Human IgG- HRP (1:5,000, Thermo Fisher Scientific, A18829) were used. .. The incubated antibodies were washed with TBST for 20 min, and signals were detected with enhanced chemiluminescence (Thermo Fisher Scientific, 34095) and Article Title: A comprehensive method for quantifying human cytomegalovirus plaque assays Article Snippet: .. The use of both smart phones and the Article Title: Suppression of neuronal p27 impairs cognitive function in middle-aged mice. Article Snippet: .. Membranes were imaged with ClarityTM ECL soluation (Bio-Rad) and the Article Title: Circadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult Mice Article Snippet: To quantify DNA loading, the same samples were loaded to the other half of the membranes and probed with anti-ssDNA antibody (MAB3034, Millipore Sigma, Burlington, MA, USA) with 1/15,000 dilution in 0.2% Tween-20 (PBS-T). .. After being incubated overnight at 4 ◦C, these were washed with PBS-T, and incubated in secondary antibody, Rabbit-anti-mouse IgG (cat. No. ab46540, Abcam, Cambridge, UK), at room temperature for 1 h. After washing, membranes were developed https://doi.org/10.3390/ijms27083408 using the Bio-Rad Western ECL kit and imaged on a Article Title: Cistanche deserticola polysaccharide alleviates bronchiectasis by regulating arginine metabolism and STAT signaling pathway. Article Snippet: The present study aimed to explore the regulatory mechanisms through which Cistanche deserticola polysaccharide (CDP) ameliorates immune-metabolic dysregulation in an experimental bronchiectasis model. Bronchiectasis was induced in six-month-old female Sprague-Dawley (SD) rats through intratracheal administration of Pseudomonas aeruginosa.. The experimental animals were divided into multiple groups: sham-operated controls, disease-induced models, low-dose and high-dose CDP treatment cohorts, as well as a positive control group treated with prednisone.. After 14 days of intervention, tissue histopathology, flow cytometry, ELISA, qPCR, metabolomics, and Western blot analysis were conducted. Article Title: Bipartite DNA binding domain of transcription factor BCL11B binds clustered short DNA sequence motifs Article Snippet: Data were analyzed using GraphPad Prism 10. .. Following the fluorescence polarization assay, 10 μL of each reaction mixture was analyzed on an 8% native polyacrylamide gel run in ice-cold 0.5× TBE buffer at 150 V for 25 min. Gels were imaged using Software:Article Title: Photobiomodulation at 830 nm with 5 J/cm 2 does not promote PI3K/AKT/mTOR signalling pathway activation in hyperglycemic wounded cells Article Snippet: .. The bands presence were scanned using a Western Blot:Article Title: Circadian Controlled Transcription in Brain and Peripheral Organs of Juvenile and Adult Mice Article Snippet: To quantify DNA loading, the same samples were loaded to the other half of the membranes and probed with anti-ssDNA antibody (MAB3034, Millipore Sigma, Burlington, MA, USA) with 1/15,000 dilution in 0.2% Tween-20 (PBS-T). .. After being incubated overnight at 4 ◦C, these were washed with PBS-T, and incubated in secondary antibody, Rabbit-anti-mouse IgG (cat. No. ab46540, Abcam, Cambridge, UK), at room temperature for 1 h. After washing, membranes were developed https://doi.org/10.3390/ijms27083408 using the Bio-Rad Western ECL kit and imaged on a Fluorescence:Article Title: Bipartite DNA binding domain of transcription factor BCL11B binds clustered short DNA sequence motifs Article Snippet: Data were analyzed using GraphPad Prism 10. .. Following the fluorescence polarization assay, 10 μL of each reaction mixture was analyzed on an 8% native polyacrylamide gel run in ice-cold 0.5× TBE buffer at 150 V for 25 min. Gels were imaged using |